FastQCFastQC Report
Tue 3 May 2016
SRR3192401_1_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3192401_1_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95236637
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1229050.048.2719761
GTATCAA2015700.034.796451
GAGTACA2310300.032.495091
AGTACAT2428600.030.214772
GTACATG3515150.030.1931441
TACATGG3600600.029.080312
CAACGCA2464000.027.8370275
ATCAACG2487850.027.46043
ACATGGG3851800.026.6723923
TCAACGC2630000.026.2627264
TATCAAC2678700.025.8922122
AACGCAG2679300.025.8696846
GTACTAG277950.025.335371
CTAGTAC372050.025.1536673
CTAACGC151850.025.1307093
CATGGGG3089150.024.5433714
TAACGCC154700.024.5261384
CTGTGCG390950.023.598019
TCTAACG162350.022.912252
TCGACGG169150.022.81936894
Produced by FastQC (version 0.11.2)